Katanin Regulates Microtubule Dynamic Instability

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Microtubule dynamic instability

What are microtubules? One of three types of protein polymer that make up the cellular cytoskeleton, an elaborate array of filaments that are used to establish cell shape, facilitate cell motility, organize organelles and assist with cell division. Microtubules are stiff tubes, about 25 nm in diameter. During interphase, they serve as tracks on which organelles and the nucleus are positioned by...

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The dynamic activities of cellular microtubules (MTs) are tightly regulated by a balance between MT-stabilizing and -destabilizing proteins. Studies in Xenopus egg extracts have shown that the major MT destabilizer during interphase and mitosis is the kinesin-related protein XKCM1, which depolymerizes MT ends in an ATP-dependent manner. Herein, we examine the effects of both overexpression and ...

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Regulation of microtubule dynamic instability.

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The completion of cytokinesis is crucial for mitotic cell division. Cleavage furrow ingression is followed by the breaking and resealing of the intercellular bridge, but the detailed mechanism underlying this phenomenon remains unknown. Katanin is a microtubule-severing protein comprised of an AAA ATPase subunit and an accessory subunit designated as p60 and p80, respectively. Localization of k...

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ژورنال

عنوان ژورنال: Biophysical Journal

سال: 2014

ISSN: 0006-3495

DOI: 10.1016/j.bpj.2013.11.1997